Factors Affecting Human Umbilical Cord Blood Quality Prior to Cryopreservation: The Importance of Birth Weight and Gestational Age

Authors: Phuong Hoang Nguyen, Van-Tinh Nguyen, Thao Thi Chu, Linh-Huyen Truong, Thu Thi Hoai Do, Tu Dac Nguyen, Anh Viet Bui, Tien Anh Ngo, Uyen Thi Trang Than, and Liem Thanh Nguyen

Introduction

Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells and is useful for the treatment of blood diseases. The cost of UCB storage is high; thus, it is necessary to evaluate the quality of UCB before collection and cryopreservation.

Objective

This study aimed to determine the maternal and neonatal factors that influence the UCB prior to selection for cryopreservation.

Methods

The analysis included 403 processed UCB units. The effects of maternal characteristics, including maternal age and delivery method, and neonatal factors, such as birth weight, gestation duration, and sex, on UCB quality were determined based on the collected blood volume, total nucleated cell (TNC) count, and CD34+ cell count.

Results

The neonatal birth weight influenced the collected blood volume, TNC count, and CD34+ cell count. Neonates with higher birth weights produced better quality UCB units because of increased collected blood volumes, TNC counts, and CD34+ cell counts. However, an increase in the gestational age from 35 to 41 weeks led to decreases in the collected blood volume and CD34+ cell count.

Discussion

These data may be useful in determining the optimal cord blood units for collection and cryopreservation as well as in advising pregnant women using private banking services.