Meaning of Hemopexin in Blood

Hemopexin plays a role in binding the heme components of hemoglobin - released during the breakdown of red blood cells. In severe cases of hemolysis, destruction of haptoglobin leads to a decrease in the patient's serum and plasma hemoglobin levels.

With the principle of scattering immunity, Hemopexin in blood samples will form an immune complex with specific antibodies present in the reagent. These complexes scatter the beam of light that passes through the sample. Then the scattered light intensity is proportional to the concentration of Hemopexin in the analyzed sample. Hemopexin quantitative test results were evaluated against a standard curve with known concentrations.

1.1. Performer, means
Carrying out quantification of Hemopexin in blood will be quantified by a doctor or technician specializing in Biochemistry with appropriate qualifications, trained in using automatic biochemical machines, and quantitatively carrying out the quantitative analysis. Facilities include:
Analyzer: BN ProSpec, ... Centrifuge Refrigerator storage: chemicals, standards, QC and patient samples Test tubes, Pipep test tube racks of all kinds, green cones , yellow consumables Consumables: blood collection tubes, needles, antiseptic cotton swabs, gloves,... 1.2. Chemical
The chemical used to perform the determination of Hemopexin in the blood is the main reagent which is the animal serum produced by immunostaining in rabbits with human Hemopexin, of high purity with an active antibody concentration of <3.5 g/L Preservative: Sodium Azide <1 g/L. Packing: 1 x 2 mL.
Chemicals are stored and stable at 2-8 degrees within the shelf life on the drug label. After opening the medicine box, it can be stored 2-8 degrees for 4 weeks provided that the lid is tightly closed after each use to avoid microbial contamination from the external environment. Take care not to freeze chemicals.
Chemicals for measuring Hemopexin blood have stability on the machine for at least 3 days with 8 hours per day per day or in the corresponding period.
1.3. Patients: Need to be specifically explained by the doctor about the purpose of the test, how to do it so that they can cooperate in the process of taking samples for testing.
1.4. Test order sheet: It is necessary to write full information of the patient such as full name, age, department, diagnosis, other information on sample status, name of doctor appointing, date and time of appointment, date and time of sampling. , drugs used (if any)...

2.Steps to proceed 2.1. Sample collection and handling
Collect blood samples from the patient specifically heparin serum and plasma samples Test samples as fresh as possible are kept at 2-8 degrees for no more than 7 days, or samples are frozen . Serum and plasma samples can be stored below -20 degrees for 3 months if samples are frozen for 24 hours after sampling to avoid freezing. For serum samples that must be completely coagulated, after centrifugation should not contain any particles or fibrin. With greasy, frozen blood samples that are cloudy after defrosting, they need to be re-clarified by centrifugation before measuring Hemopexin. 2.2 Carry out quantification of Hemopexin in blood
Set up the calibration curve: by multi-point calibration. A series of dilutions of N Protein Standard SL are performed automatically by the instrument using N Diluent. These diluent calibrators are used within 4 hours. This calibration curve is valid when the controls with the respective method, such as N/T Protein Controls SL/L, M and H, are repeated within the respective confidence intervals. A new calibration curve must be established when a new batch of reagents is used. Samples: should be automatically diluted 1:20 with N Diluent and should be measured in 4 hours. If the result is out of range, it is necessary to rerun the sample with a higher or lower dilution. Internal Control: Run N/T Protein Controls SL/L, M and H after establishing the calibration curve, before the first use of a new reagent vial, and with each run of samples. Control samples should be performed and evaluated in the same way as patient samples. Comply with regulations and quality management requirements for the frequency of internal audits. Rerun if a control result is outside the confidence interval and if the rerun results confirm a deviation, a new calibration curve should be established to obtain an accurate test result. How it works: The analyzer is prepared to perform the blood Hemopexin quantification. The meter is calibrated with the Hemopexin assay. IQC Hemopexin results are satisfactory when not outside the allowable range and do not violate the quality control law. The person performing the sample analysis test enters data about the patient information and assigns the test to the analyzer or network system (if any) Perform the analysis of the patient sample according to the protocol of the testing machine. The results need to be reviewed and evaluated before printing the report or recording the results on the test sheet to return to the patient.

3. Verification of results Quantitative hemopexin in blood has a reference value: Hemopexin: 0.5 - 1.15 g/L Hemopexin decreases in case of hemolysis. It is necessary for each laboratory to establish its own reference range as values ​​may vary from one particular population to another.

4. Errors and handling The results of quantitative testing of Hemopexin in the blood are not affected when the concentration of substances reaches: triglycerides ≤ 2.71mmol/L(2.4 g/L), bilirubin ≤ 1026 mol/L and free hemoglobin ≤10 g/L. The specimen is cloudy, and the particles in the sample affect the test results. It should be centrifuged before performing the test. Do not use greasy, cloudy, non-centrifugal blood samples again, need to take a new sample to meet the requirements. After having the results of blood hemopexin test, the doctor needs to explain in detail to the patient in combination with the medical history, clinical manifestations... to have an accurate correlation.
Quantification of Hemopexin in blood is performed at Vinmec International General Hospital system by a team of highly specialized doctors and technicians with the support of a modern testing machine system. Please book an appointment on the website (vinmec.com) for the best service.

Reference source: Ministry of Health