Meaning of the Sero Amibe test

Entamoeba histolytica is a protozoan protozoan parasite with 3 forms: the active erythrocyte-eating form, also known as the pathogenic form, which can be found in the feces of infected people. The second body is an active small body, in this form, the parasite has not eaten red blood cells, so it has not caused disease. They are usually saprophytic in the intestinal lumen and can be found in the feces of people without dysentery . The third body is the cystic form and this is the transmissible form. They are characterized by high resistance to the external environment and are a source of infection for healthy people.
Entamoeba Histolytica is the causative agent of amoeba. It is a locally endemic disease and rarely becomes an epidemic. Amoeba has 3 main types, namely acute dysentery with the pathological characteristics of rapid onset with common symptoms of painful cramps, bloody stools. The second form of Amoeba is chronic dysentery, this is a type of amoeba. chronic inflammatory bowel disease during acute amoebic dysentery. The third type is the extraintestinal amoeba, the most common being hepatic amoeba, because the amoeba migrates from the intestinal lesions through the bloodstream into the liver and causes liver abscesses. In rare cases we can also have amoebiasis of the lungs and brain abscesses.
Amoeba, when entering the body, will secrete an enzyme that destroys the intestinal lining, causing damage to the intestinal wall. In addition, they together with other bacteria in the intestinal lumen cause more severe damage to the intestinal wall. Notably, when in the intestinal wall, amoeba can also penetrate into the blood and cause diseases in other parts of the body such as liver, lungs, brain,...

Extra-colon diseases caused by amoeba are difficult to know based on symptoms alone, because these symptoms are quite similar to other diseases. Therefore, doctors often have to use the method of scanning stool samples of patients. However, this method has the limitation of low sensitivity and is usually only used in cases of patients with colonic amoeba.
Currently, for cases of extra-colonous amoeba, doctors often assign patients to have serologic tests, especially ELISA (sero amibe) immunoreactivity test, this is a The test is considered to have high diagnostic value.

Sero Amibe is a test performed for the detection of circulating Entamoeba histolytica IgG antibodies by enzyme immunoassay. The specimen used for this test is the patient's serum. The basic principle of this test method is based on a specific antigen-antibody combination reaction, in which the antibody has been attached with an enzyme. If color is present, a specific antigen-antibody reaction has occurred in the sample. The specimen has a positive test result when the antibody titer is > 1/200.
The Sero Amibe test for IgG antibodies in the blood is highly sensitive, so it can detect antibodies even at very low levels (0.1 ng/mL). Entamoeba histolytica IgG antibodies can be detected up to 1 week after the onset of symptoms of amoebic colitis. Although very rare, when conducting tests, we also need to be aware of false positives or suspected cases. In those cases, the patient should be referred for a repeat test for final results.
Patients need to go to medical facilities to perform Amibe test when infected with helminths in general or Entamoeba histolytica in particular, especially those who have a habit of eating raw vegetables, salads.... Or you should also get tested every 6 months to check if you have any helminths or protozoa that cause disease. In addition, if you see symptoms such as frequent abdominal pain, unexplained weight loss, feeling tired, pale skin, digestive disorders, headache, dizziness... you should also test Amibe sero .
It is best in case you suspect that you are infected with helminths or other protozoa that cause disease, you should go to a medical facility for examination and diagnosis of amoeba.
Vinmec International General Hospital is currently a reputable and good quality medical facility. The examination process is carried out by experts, good and experienced doctors. Along with that is a system of modern machinery and equipment that will serve the examination and treatment to achieve better results. Currently, the hospital applies conventional testing and imaging methods such as ultrasound and endoscopy to find parasites in internal organs.
In addition, other imaging techniques such as: X-ray, magnetic resonance imaging, computerized X-ray scan (CAT),... are also used to find parasites when they are suspected. causing damage to organs in the body such as the heart, lungs, brain, intestines, muscles,... Therefore, customers can be assured of the quality of service and medical care at Vinmec.