Steps to take samples for HPV test

The article was written by Doctor Tran Thi Thanh Huong - Department of Internal Medicine, Vinmec Times City International General Hospital.

HPV (Human Papilloma virus) is a common sexually transmitted virus, there are many different strains. Some strains can lead to cancer of the cervix, vulva, genitals, or cancer of the anus and penis.

Cervical cancer is one of the common diseases, common in women aged 40-60, with >95% of cervical cancer cases detected with HPV virus. People with HPV can spread the virus to others without even knowing it because they have absolutely no symptoms.

This test is done in a gynecological clinic, no samples are taken during menstruation, customers do not douche, put drugs or have sex before 24 hours. Customers have been able to urinate cleanly, change skirts and take off underwear.
Ask clients about PARA, menstrual cycle, previous laboratory history, any gynecological treatment and family history of breast or uterine cancer?
Prepare tools: speculum, gloves, physiological saline, gel, pins, gauze, swabs and swabs for HPV testing. Procedure: the client lies on the table in the gynecological position, relaxes the body. The doctor disinfects his hands, puts on gloves, places a speculum, exposes the cervix, and fixes the speculum. Observe the vaginal and cervical fluid, insert the brush into the cervix, gently press and rotate around the cervical opening for about 5 times (make sure to get all the cells in the border area) then take the brush out and dip it into the bottle containing the solution. preservative solution, press down on the bottom of the vial 10 times, then rotate the brush vigorously a few times to get the most out of the cells. Clean the vagina, remove the speculum, and instruct the client to change. Record the patient's complete and accurate information in the patient's vial, send the specimen to the laboratory. Discuss with customers after examination and answer if customers ask, guide to go to gynecological examination periodically. Make an appointment with the customer for the time to get the results and when the results are available call, consult the results for the customer or give a prescription if needed. Vinmec International General Hospital is performing routine HPV genotyping on Seegene's automated system (Korea) which has been certified CE-IVD.
This technique is based on Real-time PCR principle, has higher sensitivity, specificity and feasibility than conventional PCR in detecting HPV infection and genotyping, can simultaneously detect 28 types The difference includes all types in the low-risk and high-risk groups.

To help customers, especially female customers who are interested and have a need for HPV testing, Vinmec International General Hospital offers a basic gynecological examination and screening package designed specifically for women. (no age limit). When registering for the package, customers will receive:
Gynecological specialist examination Transvaginal ultrasound of the uterus and ovaries Transvaginal ultrasound of the breast on both sides Tests such as: Treponema pallidum rapid test, Chlamydia rapid test, specimen collection Cervical-vaginal cytology, bacteriological staining (female vaginal fluid), HPV genotype PCR automated system Total urinalysis by automatic machine

The HPV vaccine is considered safe and can be highly effective in protecting women from diseases related to HPV types 16 and 18, the two main types that cause cervical cancer. .
Therefore, doctors recommend the best vaccination from 9-26 years old, adults from 27-45 years old, women who have had sex can still be vaccinated. Currently, there are 3 types of vaccines: divalent vaccine (prevents type 16,18), quadrivalent vaccine (prevents type 6,11,16,18), and multivalent vaccine (prevents type 6,11, 16,18). 31,33,45,52,58).