Thaw sperm from extremely poor quality samples before freezing

The article was written by Doctor Diem Thi Yen - Doctor of IVF Lab, Reproductive Support Center - Vinmec Times City International Hospital

Sperm samples of extremely poor quality are samples with a sperm concentration of less than 1 million/ml of semen, or sperm obtained from surgery. With such sperm samples, a special freezing method is required, both to increase the ability.

Sperm thawing is a technique in which a sperm sample frozen and stored in a storage vessel is thawed, then filtered and washed for use.

2.1 Preparation of Defrosting Plate: contains drops of gametophyte culture medium, then covered with a layer of oil to prevent evaporation and stabilize pH and concentration of substances. Leave overnight in the incubator, at 37 degrees Celsius, 6% CO2 before use. Check the quality of sperm before freezing to decide to get the right number of stalks needed for 1 time of IVF. The embryonic stem contains frozen sperm, which is immersed in liquid nitrogen. Other necessary supporting tools.

2.2 Conduct Check the information on the patient's file, matching the information on the sperm storage tube: Full name, date of birth, PID number, date of spermatogenesis. Quickly lift the sperm storage tube from the nitrogen and dip it into the defrosting dish, so that the entire sperm-containing stalk is submerged in the drop of gamete culture. Gently shake the stem to allow the sperm to gradually dissolve into the drop of gametophyte culture medium. Take out the sperm storage stalk, store the plate in the culture cabinet, to stabilize the sperm after thawing. Reassess sperm quality according to WHO 2010 standards, and ready to use for IVF. Customers who need accurate examination and advice on sperm freezing and thawing services can choose Reproductive Support Center - Vinmec International General Hospital. The center currently gathers a team of leading experts in the fields of obstetrics and gynecology, domestic and international, and a team of doctors and nurses trained in the world's leading countries in medicine such as the US and Singapore. , Japan, Australia and world famous Assisted Reproductive Centers. With a high level of expertise and extensive experience, the team of experts at Vinmec Fertility Center are capable of synchronously and comprehensively deploying today's most advanced assisted reproductive techniques, helping to realize fulfill the parenting dream of hundreds of families across Vietnam.