Steps to thawing and isolating sperm from testicular tissue

Currently, thanks to the development of medicine, people have successfully treated many cases of azoospermia by freezing spermatozoa to preserve sperm then isolating sperm from thawed testicular tissue. to be cultured and fertilized.

Treatment of male infertility has long been a matter of concern from both the medical side and the community. Infertility can be caused by many causes such as congenital, due to infection with immune diseases or diseases affecting sperm such as mumps... or due to improper diet, living, rest ... In fact, among couples without children, there are about 1% of cases due to husband's azoospermia.
Thawing of testicular tissue is a technique to thaw testicle tissues that have been preserved by freezing sperm for a long time so that sperm can be extracted from thawed tissue and brought to culture. implanted and put into fertilization.
Thawing of testicular tissue is indicated for all cases of cryopreservation of sperm tissue that need to be thawed to retrieve sperm for use. It is also a rigorous process that requires careful execution.
According to clinical statistics, up to 70 in 100 men with infertility are due to testicular failure to produce sperm or very poor spermatogenesis. In these cases of azoospermia, the examination showed that the damaged testicular tissue was heterogeneous, besides the severely damaged tissue completely unable to produce sperm, there were still areas of seminal tissue. testicles are slightly damaged, still have the ability to produce sperm, even have sperm in them. However, due to the low sperm count, most of them die in the testicles without having time to swim out. That greatly affects the ability to conceive.
After many years of research, through observations under the microscope, people realize that they can completely find and isolate sperm from fertile tissue areas to serve the process of conception. Sperm cryopreservation is one of the methods to support this process by taking, keeping, storing and preserving testicular tissues containing sperm for a long time. Then, when necessary, those tissues are used, thawed, and sperm isolated from thawed testicular tissue, cultured, and finally injected directly into the wife's eggs for spontaneous fertilization. natural or used for artificial insemination in vitro.

Steps to defrost sperm tissue:
Remove the tube containing testicular tissue from the liquid nitrogen container and then leave it at room temperature for 15 to 30 minutes to gradually increase the temperature of the tissue. Wash testicular tissue with previously prepared washing medium. Place thawed testicular tissue in a petri dish containing a pre-prepared IVF medium and shred the testicular tissue so that sperm can be found in the tissue. Observe and evaluate the activity and motility of sperm after being extracted. Sperm culture at room temperature 37 degrees C with CO2 concentration of 5% for 24 hours. Reassess sperm motility then use for ICSL.

In the process of thawing testicular tissue, to be able to successfully fertilize, the isolation of sperm from thawed testicle tissue is an extremely important factor, which can be said to be decisive for the whole next process.
As we all know, all frozen sperm samples are taken from male patients who are having fertility problems, the most common problem here is mainly infertility cases, infertility .
The success rate of this method is relatively high because the sperm cryopreservation process is rigorous, and the sperm quality after freezing is not affected. However, this does not mean that all cases are successful the first time it is implemented. In some cases, because the number of sperm in the tissue is not enough to carry out fertilization, the whole process will be done again for the most optimal effect.

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Refrigeration of sperm, thawing of testicular tissue, isolation and separation of sperm from thawed testicle tissue, all must be carried out in a medical environment with adequate instruments, equipment and sterility assurance. , the request is made by people with high professional expertise. The success rate depends on a lot of factors.
Customers who need accurate examination and advice on defrosting and sperm isolation from testicular tissue can choose Reproductive Support Center - Vinmec International General Hospital. The center currently gathers a team of leading experts in the fields of obstetrics and gynecology, domestic and international, and a team of doctors and nurses trained in the world's leading countries in medicine such as the US and Singapore. , Japan, Australia and world famous Assisted Reproductive Centers. With a high level of expertise and extensive experience, the team of experts at Vinmec Fertility Center are capable of synchronously and comprehensively deploying today's most advanced assisted reproductive techniques, helping to realize fulfill the parenting dream of hundreds of families across Vietnam.